ER Dilation and Aberrant Zymogen Granule Phenotype in Secretory Cells of ERdj4 -/- Mice
نویسندگان
چکیده
منابع مشابه
Nucleotide sequence encoding the major glycoprotein (GP2) of rat pancreatic secretory (zymogen) granule membranes.
The major protein in the pancreatic secretory (zymogen) granule phase sequencing of purified protein. By use of the PCR product, membrane (1) is a glycoprotein (GP2) with molecular mass of two clones which contained the entire coding region of GP2 in ~ 80 KD in the rat (GP-80) and ~ 75 KD in the dog (GP-75). both orientations were purified from a rat pancreas cDNA library GP-75 was isolated fro...
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Application of the laser-based technique of photon correlation spectroscopy to an in vitro study of the ionic stability and interaction kinetics of zymogen granules isolated from rat exocrine pancreas is described here. In addition the separation from pancreatic acinar cell cytosol of a factor which stabilizes isolated zymogen granules and inhibits cation-induced granule aggregation is outlined...
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Fixation by osmium tctroxidc and glutaraldehyde of zymogcn granules isolated from rat parotid and pancreas was investigated. Protein determinations showed that osmium tctroxide caused rapid release of most of the soluble protein of the granule during fixation in buffered isotonic sucrose. Such granules when examined in the electron microscope after shadow casting appeared quite flat, indicating...
متن کاملRegulation of secretory granule pH in insulin-secreting cells.
Luminal acidification is important for the maturation of secretory granules, yet little is known regarding the regulation of pH within them. A pH-sensitive green fluorescent protein (EGFP) was targeted to secretory granules in RIN1046-38 insulinoma cells by using a construct in which the EGFP gene was preceded by the nucleotide sequence for human growth hormone. Stimulatory levels of glucose do...
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Syncollin is a 13-kDa protein associated with the membranes of pancreatic zymogen granules. Here we determine the in situ localization of syncollin in pancreatic acinar cells from adult and neonatal rats, and study the targeting of green fluorescent protein-(GFP-) and His(6)-tagged syncollin chimaeras in model exocrine and endocrine secretory cells. Immunocytochemical analysis of the distributi...
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ژورنال
عنوان ژورنال: Microscopy and Microanalysis
سال: 2011
ISSN: 1431-9276,1435-8115
DOI: 10.1017/s1431927611001942